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Fisher Scientific superscript iii reverse transcriptase
Superscript Iii Reverse Transcriptase, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/superscript+reverse+transcriptase/m+mlv+reverse+transcriptase/pmc13157194-36-0-5
Average 86 stars, based on 1 article reviews
superscript iii reverse transcriptase - by Bioz Stars, 2026-10
86/100 stars

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Isolation:

Article Title: Fisetin ameliorates vascular smooth muscle cell calcification via DUSP1-dependent p38 MAPK inhibition
Article Snippet: .. Total RNA was isolated by using Trizol Reagent and reverse transcription was performed by using oligo(dT) 12-18 primers and SuperScript III Reverse Transcriptase (all from Fisher Scientific). .. RT-PCR was performed with iQ Sybr Green Supermix (Bio-Rad Laboratories) and the primers listed below (Fisher Scientific) [ – ].

Article Title: Fisetin ameliorates vascular smooth muscle cell calcification via DUSP1-dependent p38 MAPK inhibition.
Article Snippet: .. Total RNA was isolated by using Trizol Reagent and reverse transcription was performed by using oligo(dT)12-18 primers and SuperScript III Reverse Transcriptase (all from Fisher Scientific). .. RT-PCR was performed with iQ Sybr Green Supermix (Bio-Rad Laboratories) and the primers listed below (Fisher Scientific) [56–58].

Reverse Transcription:

Article Title: Fisetin ameliorates vascular smooth muscle cell calcification via DUSP1-dependent p38 MAPK inhibition
Article Snippet: .. Total RNA was isolated by using Trizol Reagent and reverse transcription was performed by using oligo(dT) 12-18 primers and SuperScript III Reverse Transcriptase (all from Fisher Scientific). .. RT-PCR was performed with iQ Sybr Green Supermix (Bio-Rad Laboratories) and the primers listed below (Fisher Scientific) [ – ].

Article Title: Seamless, modular binary vectors for plant cell and molecular biology
Article Snippet: .. RNA was extracted from A. thaliana Col‐0 seedlings using the Spectrum Plant Total RNA (Sigma‐Aldrich) kit with on‐column DNase I digestion (New England Biolabs). cDNA was synthesized from this RNA using random hexamers and SuperScript III reverse transcriptase (Fisher Scientific). .. The H2B coding sequence was amplified with primers 5′‐caacaccagaatctggatccATGGCGAAGGCAGATAAGAAACC‐3′ and 5′‐ggagtttcagagccggatccAGAACTCGTAAACTTCGTAACCGC‐3′, the Reptin coding sequence was amplified with primers 5′‐caacaccagaatctggatccATGGCGGAACTAAAGCTATCAG‐3′ and 5′‐ggagtttcagagccggatccGATCTGCATAGCATCTTGTTCGTC‐3′, and the Pontin coding sequence was amplified with primers 5′‐caacaccagaatctggatccATGGAGAAAGTAAAGATTGAAGAAATTCA‐3′ and 5′‐ggagtttcagagccggatccTGAGATGTATTTTTCTTGTTGCTCATG‐3′ and using Phusion DNA polymerase (New England Biolabs) and purified using a Monarch Nucleic Acid Purification kit (New England Biolabs). pAVEC1::C‐mEGFP and pAVEC1::C‐mTurboID were digested with BamHI‐HF (New England Biolabs), purified using a Monarch Nucleic Acid Purification kit (New England Biolabs), and assembled with the amplified coding sequences using the one‐step T4 DNA polymerase assembly method (Islam et al ., ; Jeong et al ., ).

Article Title: Characterization and comparison of two NPC cell lines, C17 and C666-1, as models for studying the pathogenesis of nasopharyngeal carcinoma.
Article Snippet: .. SuperScript III Reverse Transcriptase was obtained from InvitrogenThermo Fisher Scientific (Darmstadt, Germany; #18080044). .. The RNeasy Mini Kit was purchased from Qiagen (Hilden, Germany; #74104).

Article Title: Recurrent mutations in the stress regulator Cap1 reveal a trade-off between azole resistance and oxidative stress response in Candida albicans
Article Snippet: .. cDNA was prepared using the SuperScript II Reverse Transcriptase (Fisher Scientific) according to the manufacturer’s instructions with oligo dT primers and 100 ng of RNA. cDNA was then diluted 1:10 with nuclease-free water for qPCR measurement. .. Realtime qPCR was conducted using the PowerUp SYBR Green Master Mix (Applied Biosystems) according to the manufacturer’s instructions to measure cDNA.

Article Title: CFIm25-dependent alternative polyadenylation in AKT2 mRNA programs macrophage polarization
Article Snippet: Anchored Oligo dT (22) primer , IDT , Cat# 51-01-15-09. .. Superscript III Reverse Transcriptase , Fisher Scientific , Cat# 18-080-093. .. Trypan Blue staining solution (0.4%) , Thermo Fisher Scientific , Cat# 15250061.

Article Title: Fisetin ameliorates vascular smooth muscle cell calcification via DUSP1-dependent p38 MAPK inhibition.
Article Snippet: .. Total RNA was isolated by using Trizol Reagent and reverse transcription was performed by using oligo(dT)12-18 primers and SuperScript III Reverse Transcriptase (all from Fisher Scientific). .. RT-PCR was performed with iQ Sybr Green Supermix (Bio-Rad Laboratories) and the primers listed below (Fisher Scientific) [56–58].

other:

Article Title: Expansion of myeloid suppressor cells and suppression of Lassa virus-specific T cells during fatal Lassa fever.
Article Snippet: Monocytes were further purified using a negative pan-monocyte selection kit with immunomagnetic separation (Miltenyi).

Synthesized:

Article Title: Seamless, modular binary vectors for plant cell and molecular biology
Article Snippet: .. RNA was extracted from A. thaliana Col‐0 seedlings using the Spectrum Plant Total RNA (Sigma‐Aldrich) kit with on‐column DNase I digestion (New England Biolabs). cDNA was synthesized from this RNA using random hexamers and SuperScript III reverse transcriptase (Fisher Scientific). .. The H2B coding sequence was amplified with primers 5′‐caacaccagaatctggatccATGGCGAAGGCAGATAAGAAACC‐3′ and 5′‐ggagtttcagagccggatccAGAACTCGTAAACTTCGTAACCGC‐3′, the Reptin coding sequence was amplified with primers 5′‐caacaccagaatctggatccATGGCGGAACTAAAGCTATCAG‐3′ and 5′‐ggagtttcagagccggatccGATCTGCATAGCATCTTGTTCGTC‐3′, and the Pontin coding sequence was amplified with primers 5′‐caacaccagaatctggatccATGGAGAAAGTAAAGATTGAAGAAATTCA‐3′ and 5′‐ggagtttcagagccggatccTGAGATGTATTTTTCTTGTTGCTCATG‐3′ and using Phusion DNA polymerase (New England Biolabs) and purified using a Monarch Nucleic Acid Purification kit (New England Biolabs). pAVEC1::C‐mEGFP and pAVEC1::C‐mTurboID were digested with BamHI‐HF (New England Biolabs), purified using a Monarch Nucleic Acid Purification kit (New England Biolabs), and assembled with the amplified coding sequences using the one‐step T4 DNA polymerase assembly method (Islam et al ., ; Jeong et al ., ).

Transformation Assay:

Article Title: Anti-transferrin receptor antibody (JST-TFR09/PPMX-T003) induces ferroptosis in adult T-cell leukemia/lymphoma (ATLL) cells.
Article Snippet: Previously, we developed a complete human IgG TFR1 antibody (JST-TFR09/PPMX-T003) that showed a potentially practical therapeutic effect against adult T-cell leukemia/lymphoma (ATLL) in vitro and in vivo.. In the present study, to elucidate the molecular mechanism underlying ATLL cell death induced by anti-TFR1 antibodies, we performed comprehensive gene expression analysis and mass spectrometry on ATLL cells treated with PPMX-T003 antibody.. These results suggest that PPMX-T003 antibody treatment of ATLL cell lines induces ferroptosis mediated by ferritin degradation.

Infection:

Article Title: Anti-transferrin receptor antibody (JST-TFR09/PPMX-T003) induces ferroptosis in adult T-cell leukemia/lymphoma (ATLL) cells.
Article Snippet: Previously, we developed a complete human IgG TFR1 antibody (JST-TFR09/PPMX-T003) that showed a potentially practical therapeutic effect against adult T-cell leukemia/lymphoma (ATLL) in vitro and in vivo.. In the present study, to elucidate the molecular mechanism underlying ATLL cell death induced by anti-TFR1 antibodies, we performed comprehensive gene expression analysis and mass spectrometry on ATLL cells treated with PPMX-T003 antibody.. These results suggest that PPMX-T003 antibody treatment of ATLL cell lines induces ferroptosis mediated by ferritin degradation.

Concentration Assay:

Article Title: Anti-transferrin receptor antibody (JST-TFR09/PPMX-T003) induces ferroptosis in adult T-cell leukemia/lymphoma (ATLL) cells.
Article Snippet: Previously, we developed a complete human IgG TFR1 antibody (JST-TFR09/PPMX-T003) that showed a potentially practical therapeutic effect against adult T-cell leukemia/lymphoma (ATLL) in vitro and in vivo.. In the present study, to elucidate the molecular mechanism underlying ATLL cell death induced by anti-TFR1 antibodies, we performed comprehensive gene expression analysis and mass spectrometry on ATLL cells treated with PPMX-T003 antibody.. These results suggest that PPMX-T003 antibody treatment of ATLL cell lines induces ferroptosis mediated by ferritin degradation.

Real-time Polymerase Chain Reaction:

Article Title: Recurrent mutations in the stress regulator Cap1 reveal a trade-off between azole resistance and oxidative stress response in Candida albicans
Article Snippet: .. cDNA was prepared using the SuperScript II Reverse Transcriptase (Fisher Scientific) according to the manufacturer’s instructions with oligo dT primers and 100 ng of RNA. cDNA was then diluted 1:10 with nuclease-free water for qPCR measurement. .. Realtime qPCR was conducted using the PowerUp SYBR Green Master Mix (Applied Biosystems) according to the manufacturer’s instructions to measure cDNA.



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